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Submitted: 26 September 2013 Modified: 26 September 2013

Herdin Record #: R11-DMSF-09261311092814

Acute Toxicity Dose in Mice, Approximate Effective Dose, Effective Dose (ED50) and Bioassay of Calabash (Crescentia cujete) Fruit Decoction as a Hypoglycemic Agent in Alloxan-induced Hyperglycemic Rabbits


1Haris  Rashid Author
2Herminigilda  B. Nartatez Adviser
3Michael Jan  Pimentel Author
4Eva  C. San Juan Adviser
5John Edward  C. Padisio Author
6Melinda  C. Tagle Adviser
7Chris  Dy Author
8Genevieve  D. Tupas Adviser
9Sittie Soriena  D. Kundo Author
10Ruth Kristine  Tan Author
11Rechie Mae C. Abear Author
12Rodessa  . Tabunda Author
13Muhammad Razul  Abdullah Author
14Sittie Jebrailyn  Amilhasan Author
15Rus Cyan Josef  E. Rodelas Author

Publication Information

Publication Type:
Publication Sub Type:
Research Journal of the Davao Medical School Foundation, Inc
Publication Date:
Davao Medical School Foundation, Inc.


Diabetes ranks fourth in the leading causes of mortality worldwide and eighth leading cause of death in the Philippines. Treatment options for diabetes are being expanded beyond the practice of conventional medicine, with the increasing popularity and use of herbal medicines. This study was conducted to investigate folkloric claim on the potential of calabash (Crescentia cujete) decoction in reducing blood glucose level. The study aimed to determine the acute toxicity dose, approximate effective dose (aed) and effective dose (ed50) of calabash fruit decoction and test this in a bioassay using Metformin and Normal Saline Solution (nss) as positive and negative controls, respectively. To test for acute toxicity, 2,000mg/kg and 5,000mg/kg doses of the calabash decoction were tested in mice. To determine the aed and ed50, alloxan was used to induce hyperglycemia in rabbits. The results showed that all mice survived the 2,000 and 5,000mg/kg doses of the calabash decoction leading to the fruit decoction’s classification as Category 5 on the Global Harmonized System of Classification and Labelling of Chemicals rendering it as non-toxic. aed was noted to range from 3.98 to 15.84 mg/kg. ed50 Probit analysis of the fruit decoction was determined at 9.88 or 10 mg/kg. Experimental bioassay showed the mean blood glucose levels at 283.00, 261.87 and 241.47mg/dl for NSS, Metformin, and calabash decoction, respectively. Evaluation using Post Hoc Pair-wise test demonstrated that calabash and Metformin had comparable hypoglycemic effect. In conclusion, this study showed that the calabash fruit decoction is non-toxic and is effective in lowering the blood glucose levels of alloxan-induced hyperglycemic rabbits at a dose of 10mg/kg. Its hypoglycemic effect is similar to Metformin.

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